Merck 109204 2500 Giemsa’S Azur Eosin Methylene Blue Solution For Microscopy 2,5 LT.

Merck 109204 2500 Giemsa’S Azur Eosin Methylene Blue Solution For Microscopy 2,5 LT.

114.54 ( KDV Dahil )

for microscopy

Stok kodu: 109204.2500 Kategoriler: ,


Catalogue Number 109204
Description Giemsa’s azur-eosin-methylene blue solution
Overview Giemsa’s Azure Eosin Methylene Blue solution – for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y – Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Product Information
HS Code 3212 90 00
Quality Level MQ400

Application for microscopy

Physicochemical Information
Boiling point >65 °C (1013 hPa)
Density 0.99 g/cm3 (20 °C)
Explosion limit 5.5 – 44 %(V) Methanol
Flash point 18 °C
Ignition temperature 455 °C
pH value 6.0 – 8.2 (H₂O, 20 °C) (undiluted)

Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s) H225: Highly flammable liquid and vapour.
H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
H317: May cause an allergic skin reaction.
H370: Causes damage to organs.
Precautionary Statement(s) P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal Word Danger
Storage class 3 Flammable liquids
WGK WGK 2 obviously hazardous to water
Disposal 9
Carcinogenic compounds and flammable compounds labelled “Highly toxic” or “Toxic”: container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).

Safety Information
Categories of danger highly flammable, toxic

Storage and Shipping Information
Storage Store at +15°C to +25°C.

Transport Information
Declaration (railroad and road) ADR, RID UN 1230 , 3 (6.1), II
Declaration (transport by air) IATA-DGR UN 1230 , 3 (6.1), II
Declaration (transport by sea) IMDG-Code UN 1230 , 3 (6.1), II

Suitability for microscopy passes test
Erythrocytes reddish-brownish
Nuclei / Chromatin red to violet
Eosinophilic granules reddish to red-brown
Neutrophilic granules light violet
Lymphocyte cytoplasmn blue
Monocyte cytoplasm grey-blue
Thrombocytes violet
Basophilic granules dark violet
Nuclei (Tissue section) blue to dark blue
Cells blue to dark blue
Collagen pale blue
Osteoid pale blue
Eosinophilic granula (Tissue section) red
Acidic mucopolysaccharides reddish-violet
Mast cell granules reddish-violet
Cartilage matrix reddish-violet
Acidophilic materials orange-red
Helicobacter pylori blue to dark blue
blue to dark blue
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